DISTURBANCE OF PRO-OXIDANT-ANTIOXIDANT BALANCE IN RATS WITH CEREBRAL ISCHEMIA

Excess of reactive oxygen can lead to membrane damage, accumulation lipid, protein, and nucleic acid oxidation products, deficiency reduced pyridine nucleotides phospholipids mitochondrial membranes, then electrolyte imbalance, swelling, uncoupling phosphorylation processes, ischemic neuronal death. Thus, the study oxidative stress antioxidant system activity is relevant.
 The aim examine changes in pro-oxidant-antioxidant balance rats with brain damage different degrees severity (subtotal total cerebral ischemia).
 Materials Methods. experiments were performed on 30 male outbred white weighing 260±20 g compliance requirements Directive European Parliament Council Union No. 2010/63/EU September 22, 2010 protection animals used for scientific purposes.
 Results. A more significant decrease content SH-groups proteins glutathione (by 58 (51; 64) % (p<0.05)), GSH concentration 29 (19; 35) (p<0.05)) was observed under 24-hour subtotal ischemia (SBI) compared 1-hour SBI. Changes peroxidase multidirectional: SBI, increased by 12 (9; 18) (p<0.05compared control level, it decreased 74 (67; 81) (p<0.05). In higher 60 (54; 65) (p<0.05), 42 (39; 56) (p<0.05) (TBI). products that react thiobarbituric 59 63) 24-hout 36 (29; 45) 63 (59; 75) TBI. 83 (78; 91) TBI equal zero.
 Conclusions. most pronounced disturbances Similar, but less